Evaluating an in vitro culture system of bovine uterine and oviduct epithelial cells for subsequent embryo co-culture [cows; cattle]
1992
Thibodeaux, J.K. | Goodeaux, L.L. | Roussel, J.D. | Broussard, J.R. (Louisiana State Univ., Baton Rouge (USA). Dept. of Dairy Science) | Myers, M.W. | Godke, R.A. (Louisiana State Univ., Baton Rouge (USA). Dept. of Animal Science) | Menezo, Y. (IRH, Villeurbanne (France). Foundation Merieux and INSA)
Three experiments were conducted using 5 different culture media to evaluate the effects of culture medium and incubation temperature on bovine uterine and oviduct epithelial cell growth, so that the most efficient combination could then be used to develop a co-culture system for bovine embryos. The results showed that the culture medium did not influence fertilization or cleavage rates. However, more embryos co-cultured with oviduct epithelial cells were considered viable after 7 days of incubation compared with embryos incubated in medium alone. These results indicate that various incubation conditions can influence the growth of bovine uterine and oviduct epithelial cells in vitro. However, in spite of changes in cell growth patterns, there does not appear to be a change in their embryotropic capabilities in vitro.
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