Aggregated forms of heparin-binding and non-heparin binding proteins of boar seminal plasma and their binding properties
1999
Manaskova, P. | Meszarosova, A. | Jonakova, V. (Akademie Ved, Prague (Czech Republic). Ustav Molekularni Genetiky) | Liberda, J. | Voburka, Z. | Ticha, M.
Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding (H-) proteins. Gel chromatography of the H+ portion yielded four main protein fractions of 150, 45, 30 and 20 kDa, while that of the H- portion resulted in the separation into three main protein fractions of 150, 30 and 20 kDa. HPLC analysis and N-terminal sequencing used to characterize the composition of the protein fractions obtained by gel chromatography revealed that all consisted of low (12-16 kDa) molecular weight. The high molecular weight values of fractions obtained by gel chromatography thus suggest that the proteins are present in boar seminal plasma in the form of aggregates.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Library of Antonin Svehla