Aggregated forms of heparin-binding and non-heparin binding proteins of boar seminal plasma and their binding properties
1999
Manaskova, P. | Meszarosova, A. | Jonakova, V. (Akademie Ved, Prague (Czech Republic). Ustav Molekularni Genetiky) | Liberda, J. | Voburka, Z. | Ticha, M.
Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding (H-) proteins. Gel chromatography of the H+ portion yielded four main protein fractions of 150, 45, 30 and 20 kDa, while that of the H- portion resulted in the separation into three main protein fractions of 150, 30 and 20 kDa. HPLC analysis and N-terminal sequencing used to characterize the composition of the protein fractions obtained by gel chromatography revealed that all consisted of low (12-16 kDa) molecular weight. The high molecular weight values of fractions obtained by gel chromatography thus suggest that the proteins are present in boar seminal plasma in the form of aggregates.
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