Seed and in vitro propagation of Cistus creticus

Pela, Z.

Seed and in vitro propagation of Cistus creticus

1996

Pela, Z.

To efficiently propagate Cistus creticus seeds, characterized by seed coat dormancy, several pre and post germination treatments were applied. High temperature pre treated seeds at 80, 100, 120 C., and 150 C, for 5-30 min, followed by incubation in plastic petri dishes at 10, 15 or 20 C, with or without light, for 7 weeks, resulted in 79 per cent 30 min pretreatment at 80 C, incubation at 20 C in dark, 90 per cent 5 min pretreatment at 100 C, incubation at 20 C in dark, and 72 per cent 5 min pretreatment at 150 in dark germination rates, respectively. Soaking of seeds in boiling water for 35 sec or 1 min showed the highest rates of germination by 96 per cent or 74 per cent within 4 days, respectively. Scarification with sulfuric acid 10, 15, or 30 min followed by incubation at 2o C, resulted in only 25, 17 and 26 per cent germination, respectively.

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