Light and video microscopy
2009
Wayne, Randy
"When one looks at a specimen with a light microscope, how does one know what is real and what is an illusion created by the apparatus itself? Light and Video Microscopy takes the reader step by step through philosophy, psychology, and particularly geometrical and physical optics so that the reader can interpret images formed by light microscopes. The reader can then use this knowledge to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine physico-chemical properties like the mass of the specimen, the Ca[superscript 2+] concentration or pH of the specimen and structural properties, including the alignment of macromolecules and the localization of genes or proteins in the specimen. The reader also learns how the microscope can be interfaced with a computer to image cells using digital techniques that are able to replace or augment optical techniques. One comes away from reading this book empowered to use past, present, and future microscopical techniques to visualize the microscopic world and find the real nature of life in the image."--Jacket.
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