Chemical, physical, and histological characteristics of sorghum grain as related to wet milling properties
1971
Norris, J. R (John Robert)
Sorghum grain varying widely in endosperm type, texture, and kernel size was produced at two locations in Texas during 1967. The wide genetic diversity of the sorghums selected for study enabled determination of the effect of endosperm type, texture, and kernel size on wet milling properties. Lack of a rapid, accurate method for starch analysis prompted investigation of the use of dimethyl sulfoxide (DMSO) solubilization of the starch combined with glucoamylase hydrolysis of starch to glucose. DMSO solubilization-glucoamylase hydrolysis of starch was inferior to polarimetric or acid hydrolysis techniques of starch analysis. An increase of solubilization or incubation time did not increase the low starch values resulting from use of this technique; however, decreasing the particle size of the ground sorghum sample increased the amount of starch determined. The most accurate technique for determination of starch resulted from thermal gelatinization of the ground sorghum sample followed by glucoamylase hydrolysis of the gelatinized starch. Length of incubation time and particle size had no effect on the amount of starch determined. The method of thermal gelatinization, autoclave or boiling water bath, significantly influenced the amount of starch determined, with autoclaving being the preferred method. Combination of this technique with the glucose oxidase analysis of glucose resulted in an efficient and accurate method of determining starch. ...
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