Amelioration of aluminum phytotoxicity in Solanum lycopersicum by co-inoculation of plant growth promoting Kosakonia radicincitans strain CABV2 and Streptomyces corchorusii strain CASL5

Aluminum (Al) toxicity is the main constraint for crop cultivation in acidic soils. In this study, Al-tolerant rhizobacteria Kosakonia radicincitans (CABV2) and actinobacteria Streptomyces corchorusii (CASL5) were isolated from Beta vulgaris rhizosphere in acidic soil. Both isolates displayed high tolerance to Al (10 mM), produce siderophores, indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate and solubilize phosphate. Co-inoculation of CABV2 and CASL5 strains were significantly increased the root length (312.90%), shoot length (183.19%), fresh weight (224.82%), dry weight (309.25%) and photosynthetic pigments (chlorophyll a 279.69%, chlorophyll b 188.23% and carotenoids 158.20%) of Solanum lycopersicum plants under 300 mg Al kg⁻¹ soil conditions as compared to uninoculated Al stressed plants. Similarly, the co-inoculation treated plants subjected to Al stress condition enhanced the uptake of essential nutrients (N 229%, P 252%, K 115%, Fe 185%, Mg 345% and Ca 202%) by plants as compared to Al stressed uninoculated plants. Under Al stress (300 mg Al kg⁻¹ soil), co-inoculation significantly decreased malondialdehyde content (66%), and increased catalase (83%), superoxide dismutase (82%), peroxidase (89%) activities and root exudates (organic acids 6.44–12.36 fold) in S. lycopersicum as compared to uninoculated plants, indicating that the CABV2 and CASL5 strains were reduced Al-induced oxidative stress. Moreover, co-inoculation significantly reduced Al accumulation in the root (89%), stem (95%) and leaves (94%) of S. lycopersicum under Al stress at 300 mg Al kg⁻¹ soil, compared to the uninoculated plants. This is the first report of K. radicincitans strain CABV2 and S. corchorusii strain CASL5 potentially reducing Al uptake in S. lycopersicum.