A Novel Ribonuclease with Antiproliferative Activity from Fresh Fruiting Bodies of the Edible Mushroom Lyophyllum shimeiji
2010
Zhang, R. Y | Zhang, G. Q | Hu, D. D | Wang, H. X | Ng, T. B
A 14.5-kDa ribonuclease, with an optimum pH of 6 and a temperature optimum at 70°C, was isolated from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji. It was purified by ion exchange chromatography on DEAE cellulose, Q Sepharose, and SP Sepharose, followed by FPLC gel filtration on Superdex 75, and was adsorbed on all three ion exchangers. It showed the highest ribonucleolytic potency toward poly (U), 25% as much activity toward poly (C), and undetectable activity toward poly (A) and poly (G). Its ribonucleolytic activity at 100°C was similar to that at 20°C. It suppressed proliferation of hepatoma HepG2 cells and breast cancer MCF7 cells with an IC₅₀ of 10 and 6.2 μM, respectively. It inhibited the activity of HIV-1 reverse transcriptase with an IC₅₀ of 7.2 μM.
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