Inherently fluorescent and porous zirconia colloids: preparation, characterization and drug adsorption studies
2015
Naszályi Nagy, L. | Mihály, J. | Polyak, A. | Debreczeni, B. | Császár, B. | Szigyártó, I. Cs | Wacha, A. | Czégény, Zs | Jakab, E. | Klébert, Sz | Drotár, E. | Dabasi, G. | Bóta, A. | Balogh, Lajos | Kiss, É
Porous, fluorescent zirconia particles of nearly 380 nm diameter were prepared without template molecules or labeling dyes. The porous structure is the result of aggregation-induced particle formation. The inherent fluorescence is assigned to coordinatively unsaturated Zr⁴⁺ ions at the sol–gel derived ZrO₂ surface. After physico-chemical characterization of the native zirconia particles carboxyl and/or amine bearing drug molecules (d,l-α-difluoromethylornithine – DFMO, ursolic acid – UA and doxorubicin – DOX) were adsorbed onto their surface, and the products were analyzed with Fourier-transform infrared spectroscopy (FTIR), thermogravimetry (TG), small-angle X-ray scattering (SAXS), fluorimetry and zeta potential vs. pH measurements. We have found that DOX complexes coordinatively unsaturated Zr⁴⁺ ions without dislocating them, while carboxyl-bearing drugs interact with basic surface Zr–OH sites eliminating some of the carbonate species. The adsorption of UA at the zirconia surface shifts considerably the isoelectric point of the surface and thus provides kinetic stability to the particles at physiological pH. An in vivo biodistribution study in two healthy dogs performed by SPECT/CT detection after ⁹⁹ᵐTc labeling of the nanocarriers has shown the possibility of drug delivery application.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Agricultural Library
