Direct gene transfer to protoplasts of Arabidopsis thaliana

Karesch, H.; Bilang, R.; Scheid, O.M.; Potrykus, I.

Direct gene transfer to protoplasts of Arabidopsis thaliana

1991

Karesch, H. | Bilang, R. | Scheid, O.M. | Potrykus, I.

We performed a series of direct gene transfer experiments with protoplasts of Arabodopsis thaliana ecotype Zurich. An average of more than 100 transformants were selected per 10(6) treatedprotoplasts. Stable transformation was confirmed by integration of the marker gene into high molecular weight DNA and by its genetic transmission to subsequent offspring generations.

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