Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina
1991
Maerz, M. | Sitte, P.
Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence ofHoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated fromrestriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I andSal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I andII, and apocytochrome b were localized on this map using Southern blot hybridization with heterologousgene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P.salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophyticalgae, should be valuable for taxonomic and phylogenetic studies.
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