Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

Maerz, M.; Sitte, P.

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

1991

Maerz, M. | Sitte, P.

Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence ofHoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated fromrestriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I andSal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I andII, and apocytochrome b were localized on this map using Southern blot hybridization with heterologousgene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P.salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophyticalgae, should be valuable for taxonomic and phylogenetic studies.

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Mots clés AGROVOC

algae apoproteins centrifugation chromosome mapping circular cytochrome b dna dna dna dna eukaryota evolution genes genetics mitochondrial dna ribosomal rna rna

Informations bibliographiques

Publié dans

Plant molecular biology

Volume 16 Numéro 4 Pagination 593 - 600 ISSN 0167-4412

Editeur

CSIRO Publishing

D'autres materias

Algae and seaweeds; Cytochrome-c oxidase; Mitochondrial; Nadh dehydrogenase; Ribosomal; Density gradient; Isolation & purification; Apocytochrome b; Restriction mapping; 5s; Ribosomal; Restriction mapping

Langue

anglais

Note

2019-12-05

Type

Text; Journal Article

Sur AGRIS depuis: 2024-02-28

Format: MODS

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