Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens

Kim, C.J.; Nagaraja, K.V.; Pomeroy, B.S.

Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens

1991

Kim, C.J. | Nagaraja, K.V. | Pomeroy, B.S.

An ELISA was developed and tested for its ability to detect antibodies against Salmonella enteritidis in chickens. Various features of the ELISA were evaluated and optimized. The outer membrane protein antigens selected by use of the protein immunoblotting method made the assay specific and sensitive. The assay was evaluated in chickens experimentally infected with S enteritidis. Blood samples collected at weekly intervals after experimental infection with S enteritidis were analyzed by ELISA. Results of the ELISA were compared with those of conventional serum plate and microagglutination tests. The ELISA was more sensitive and specific in the detection of S enteritidis infection than the other 2 conventional tests.

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Palabras clave de AGROVOC

animal animals antibodies antigens antigens blood chickens chickens diagnosis electrophoresis immunoblotting immunology microbiology salmonella enteritidis salmonella enteritidis salmonellosis screening

Información bibliográfica

Publicado en

American journal of veterinary research

Volumen 52 Edición 7 Paginación 1069 - 1074 ISSN 0002-9645

Editorial

Elsevier B.V.

Otras materias

Predictive value of tests; Poultry diseases; Reproducibility of results; Polyacrylamide gel; Salmonella infections; Bacterial; Bacterial outer membrane proteins; Enzyme-linked immunosorbent assay; Bacterial; Enzyme-linked immunosorbent assay

Idioma

Inglés

Nota

2019-12-04

Tipo

Text; Journal Article

En AGRIS desde: 2024-02-28

Formato: MODS

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Este registro bibliográfico ha sido proporcionado por National Agricultural Library

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