Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens

Kim, C.J.; Nagaraja, K.V.; Pomeroy, B.S.

Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens

1991

Kim, C.J. | Nagaraja, K.V. | Pomeroy, B.S.

An ELISA was developed and tested for its ability to detect antibodies against Salmonella enteritidis in chickens. Various features of the ELISA were evaluated and optimized. The outer membrane protein antigens selected by use of the protein immunoblotting method made the assay specific and sensitive. The assay was evaluated in chickens experimentally infected with S enteritidis. Blood samples collected at weekly intervals after experimental infection with S enteritidis were analyzed by ELISA. Results of the ELISA were compared with those of conventional serum plate and microagglutination tests. The ELISA was more sensitive and specific in the detection of S enteritidis infection than the other 2 conventional tests.

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Mots clés AGROVOC

animal animals antibodies antigens antigens blood chickens chickens diagnosis electrophoresis immunoblotting immunology microbiology salmonella enteritidis salmonella enteritidis salmonellosis screening

Informations bibliographiques

Publié dans

American journal of veterinary research

Volume 52 Numéro 7 Pagination 1069 - 1074 ISSN 0002-9645

Editeur

Elsevier B.V.

D'autres materias

Poultry diseases; Enzyme-linked immunosorbent assay; Reproducibility of results; Bacterial outer membrane proteins; Polyacrylamide gel; Bacterial; Predictive value of tests; Enzyme-linked immunosorbent assay; Salmonella infections; Bacterial

Langue

anglais

Note

2019-12-04

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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