Partial purification and characterization of RalF40I, a class II restriction endonuclease from Ruminococcus albus F-40, which recognizes and cleaves 5'-/GATC-3'
1998
Miyagi, T. | Javorsky, P. | Pristas, P. | Karita, S. | Sakka, K.
Restriction endonuclease RalF40I was purified from cell-free extracts of the rumen cellulolytic bacterium Ruminococcus albus F-40 by heparin-Sepharose chromatography. The preparation was active only on DNA substrates that were not Dam-methylated. RalF40I recognizes the 4-bp palindrome. 5'-/GATC-3', and cleaves DNA at the 5' side of G in the sequence, producing 5' tetranucleotide protruding ends. RalF40I is a class II restriction endonuclease and an isoschizomer of MboI and DpnII.
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