Partial purification and characterization of RalF40I, a class II restriction endonuclease from Ruminococcus albus F-40, which recognizes and cleaves 5'-/GATC-3'
1998
Miyagi, T. | Javorsky, P. | Pristas, P. | Karita, S. | Sakka, K.
Restriction endonuclease RalF40I was purified from cell-free extracts of the rumen cellulolytic bacterium Ruminococcus albus F-40 by heparin-Sepharose chromatography. The preparation was active only on DNA substrates that were not Dam-methylated. RalF40I recognizes the 4-bp palindrome. 5'-/GATC-3', and cleaves DNA at the 5' side of G in the sequence, producing 5' tetranucleotide protruding ends. RalF40I is a class II restriction endonuclease and an isoschizomer of MboI and DpnII.
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书目信息
FEMS microbiology letters
卷
164
期
1
页码
215
- 218
ISSN
0378-1097
出版者
American Dairy Science Association
其它主题
Restriction endonucleases; Isolation & purification; Growth & development; Type ii site-specific; Deoxyribonucleases
语言
英语
类型
Journal Article; Text
2024-02-28
MODS