Primary Culture of Chicken Hepatocytes as an in Vitro Model for Determining the Influence of Dioxin
2001
HOU, De-Xing | KUNITAKE, Tomohiro | KUSUDA, Jun | Fujii, Makoto
An easy method for primary culture of chicken hepatocytes was developed to study the influence of dioxin on birds. Chicken hepatocytes could maintain gene expression and protein secretion of albumin for a long period in serum-free medium with free atmosphere exchange at 37°C. Moreover, the cells showed a sensitive response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by monitoring the expression of P450 1A, theta GST (θ-GST) and albumin genes.
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