Microbispora hainanensis sp. nov., isolated from rhizosphere soil of Excoecaria agallocha in a mangrove

Xu, Xiao-Xiong; Wang, Hai Long; Lin, Hai-Peng; Wang, Cheng; Qu, Zhi; Xie, Qing-Yi; Ruan, Ji-Sheng; Hong, Kui

Microbispora hainanensis sp. nov., isolated from rhizosphere soil of Excoecaria agallocha in a mangrove

2012

Strain 211020T was isolated from rhizosphere soil of Excoecaria agallocha in a mangrove in Hainan, China. The strain produced longitudinal pair spores branching from aerial hyphae. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Microbispora, exhibiting the highest 16S rRNA gene sequence similarity (98.75 %) to Microbispora corallina JCM 10267T with a low DNA–DNA relatedness value (13±0.6 %). The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H2) and MK-9(H0), and the major fatty acids were iso-C16 : 0, iso-C15 : 0 and C17 : 0. The phospholipid profile of strain 211020T comprised phosphatidylinositol mannoside, phosphatidylethanolamine, diphosphatidylglycerol and phospholipids of unknown structure containing glucosamine. The DNA G+C content was 70.8 mol%. On the basis of phenotypic and genotypic data, strain 211020T can be distinguished as a novel species of the genus Microbispora, for which the name Microbispora hainanensis sp. nov., is proposed. The type strain is 211020T ( = CGMCC 4.5595T = DSM 45428T).

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