Stoichiometry of Reconstituted High-Density Lipoproteins in the Hydrated State Determined by Photon Antibunching
2011
Ly, Sonny | Petrlova, Jitka | Huser, Thomas | Fore, Samantha | Gao, Tingjuan | Voss, John | Laurence, Ted A.
Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obtained by photon-counting histogram analysis. Both the photon antibunching and photon-counting analyses show that reconstituted high-density lipoprotein particles contain two apo A-I molecules, which is in agreement with the commonly accepted double-belt model.
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