Bioorthogonal site-specific labeling of the 5′-cap structure in eukaryotic mRNAs
2014
Holstein, Josephin Marie | Schulz, Daniela | Rentmeister, Andrea
We present a chemo-enzymatic approach for site-specific labeling of 5′-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5′-cap which could be further modified using strain-promoted azide–alkyne cycloaddition (SPAAC).
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