Bioorthogonal site-specific labeling of the 5′-cap structure in eukaryotic mRNAs
2014
Holstein, Josephin Marie | Schulz, Daniela | Rentmeister, Andrea
We present a chemo-enzymatic approach for site-specific labeling of 5′-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5′-cap which could be further modified using strain-promoted azide–alkyne cycloaddition (SPAAC).
显示更多 [+] 显示较少 [-]AGROVOC关键词
书目信息
出版者
The Royal Society of Chemistry
其它主题
Moieties; Cycloaddition reactions
语言
英语
类型
Journal Article; Text
2024-02-28
MODS