Flow cytometric data analysis of circulating progenitor cell stability
2017
Mahar, Ernestine A. | Mou, Liping | Hayek, Salim S. | Quyyumi, Arshed A. | Waller, Edmund K.
A recent publication by Mekonnen et al. demonstrated that among women with non-obstructive coronary artery disease, higher levels of circulating progenitor cells in the blood (CPC), were associated with impaired coronary flow reserve [1].We performed a quality control assessment of the stability of circulating blood progenitor cells in blood samples stored at 4°C, to determine the time period during which blood samples can be analyzed and yield consistent data for progenitor cell content. Healthy volunteers (n=6) were recruited and underwent phlebotomy, and blood was stored in EDTA tubes at 4°C. Flow cytometry was performed to quantitate progenitor cell subsets at 0–4h, 24h, and 48h post phlebotomy. All processed samples were fixed with 1% Paraformaldehyde and 1,000,000 total data events were collected. We found no significant differences in PC data for both CD34+ (P=0.68 for one-way ANOVA) and CD34+/CD133+ (P=0.74 for one-way ANOVA).
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