Holo- and hemimetabolism of aquatic insects: Implications for a differential cross-ecosystem flux of metals

Increased metal concentrations in aquatic habitats come as a result of both anthropogenic and natural sources. Emerging aquatic insects that play an indispensable role in these environments, transferring resources and energy to higher trophic levels in both aquatic and terrestrial habitats, may inadvertently also act as biovectors for metals and other contaminants. This study measured levels of 22 different metals detected in biofilm, aquatic and terrestrial life stages of Trichoptera and Odonata, as well as riparian spiders, to examine the uptake and transfer from freshwater to terrestrial ecosystems. We show that emerging insects transfer metals from aquatic to terrestrial ecosystems, however with large losses observed on the boundary of these two environments. Significantly lower concentrations of most metals in adult insects were observed in both hemimetabolous (Odonata) and holometabolous insect orders (Trichoptera). In holometabolous Trichoptera, however, this difference was greater between aquatic life stages (larvae to pupae) compared to that between pupae and adults. Trophic transfer may have also played a role in decreasing metal concentrations, as metal concentrations generally adhered to the following pattern: biofilm > aquatic insects > terrestrial invertebrates. Exceptions to this observation were detected with a handful of essential (Cu, Zn, Se) and non-essential metals (Cd, Ag), which measured higher concentrations in adult aquatic insects compared to their larval counterparts, as well as in aquatic and terrestrial predators compared to their prey. Overall, all metals were found to be bioavailable and biotransferred from contaminated waters to terrestrial invertebrates to some degree, suggesting that risks associated with metal-contaminated freshwaters could extend to terrestrial systems through the emergence of these potential invertebrate biovectors.