Label-free silicon quantum dots as fluorescent probe for selective and sensitive detection of copper ions
2014
Zhao, Jiangna | Deng, Jianhui | Yi, Yinhui | Li, Haitai | Zhang, Youyu | Yao, Shouzhuo
In this work, label-free silicon quantum dots (SiQDs) were used as a novel fluorescence probe for the sensitive and selective detection of Cu²⁺. The fluorescence of the SiQDs was effectively quenched by H2O2 from the reaction of ascorbic acid with O2, and hydroxyl radicals from Fenton reaction between H2O2 and Cu⁺. The fluorescence intensity of SiQDs was quenched about 25% in 15min after the addition of H2O2 (1mM). While the SiQDs was incubated with AA (1mM) and Cu²⁺ (1µM) under the same conditions, the fluorescence intensity of SiQDs decreased about 55%. Obviously, the recycling of Cu²⁺ in the test system may lead to a dramatical decrease in the fluorescence of SiQDs. Under the optimized experimental conditions, the rate of fluorescence quenching of SiQDs was linearly dependent on the Cu²⁺ concentration ranging from 25 to 600nM with the limit of detection as low as 8nM, which was much lower than that of existing methods. Moreover, the probe was successfully applied to the determination of Cu²⁺ in different environmental water samples and human hair.
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