Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean
2016
Qiang, Yuan | Yun-min, Wei | Ming-ming, Fu | You-wen, Qiu | Hong-tao, Wen | Ming-hui, Zhang | Ying, Liu | Jin-xia, Ao
A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L−1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.
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