Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo
2008
Carvallo-Chaigneau, Francisco | Trejo-Solís, Cristina | Gómez-Ruiz, Celedonio | Rodríguez-Aguilera, Ernesto | Macías-Rosales, Lucía | Cortés-Barberena, Edith | Cedillo-Peláez, Carlos | Gracia-Mora, Isabel | Ruiz-Azuara, Lena | Madrid-Marina, Vicente | Constantino-Casas, Fernando
The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4'-dimethyl-2,2'-bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.
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