Designing bifunctionalized gold nanoparticle for colorimetric detection of Pb²⁺ under physiological condition
2012
Zhu, Derong | Li, Xiaokun | Liu, Xia | Wang, Jine | Wang, Zhenxin
In the present work, a kind of peptide functionalized gold nanoparticle (AuNP) has been synthesized and employed for colorimetric detection of Pb²⁺ in both aqueous solution and living cell. The AuNPs are capped by two peptide ligands: glutathione (GSH) and pentapeptide (CALNN). The GSH is used as a functional group for selectively sensing Pb²⁺ by coordination reaction, and CALNN is employed as a stabilize ligand for improving the stability of AuNPs under physiological condition, respectively. The AuNP enables to strongly interact with Pb²⁺ that leads to distinct color change of solution. Under the optimized molar ratio of GSH to CALNN on the AuNP surface, the colorimetric assay for detecting Pb²⁺ in living cell downs to 2.9fmol Pb²⁺per cell (3 times of standard deviation, 3σ) with linear relationship from 2.9 to 37.7fmol Pb²⁺ per cell. In addition, the method also shows highly selective detection toward Pb²⁺ against other common metal ions in both aqueous solution and living cell.
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