Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
2021
Wu, Shanshan | Liu, Xiyang | Ye, Shenglong | Liu, Jianmin | Zheng, Wei | Dong, Xue | Yin, Xiushan
RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19¹,², which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.
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