Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography
2009
Skalicka-Woźniak, Krystyna | Mroczek, Tomasz | Garrard, Ian | Głowniak, Kazimierz
A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds - alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds.
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