Sporulation of Bacillus popilliae in liquid cultures
1972
Haynes, W.C. | Weih, L.J.
In 1966 Haynes and Rhodes reported that Bacillus popilliae NRRL B-2309S formed spores in liquid J-medium containing activated carbon. Although yields of a few hundred thousand spores per milliliter were ultimately obtained, the numbers in subsequent experiments diminished. We reversed this trend by using inocula composed of spores formed in successful experiments. The spores used had been stored as dry films for as long as 11.5 months. Inasmuch as Haynes and Rhodes (1969a) showed that the resistant cells formed early in the sporulation cycle are probably pre- or immature spores, we were interested in comparing their efficacy in evoking sporulation with that of mature spores from later stages of the cycle. We found that early cycle forms engender relatively few refractile spores, whereas mature spores yielded as many as 31 × 106/ml. Better than 90% of the spores were accompanied in sporangia by typical refractile parasporal bodies.
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