Novel fluorescent probe for rapid and ratiometric detection of β-galactosidase and live cell imaging
2019
Chen, Xiangzhu | Zhang, Xueyan | Ma, Xiaodong | Zhang, Yuanyuan | Gao, Gui | Liu, Jingjing | Hou, Shicong
β-Galactosidase (β-gal) is an important biomarker for primary ovarian cancers and cell senescence; however, a fast response fluorescent probe for ratiometric monitoring is still rare. A novel, ratiometric water-soluble fluorescent probe (FLM) for β-gal was developed. The emission ratio F550/F450 reached the maxima at about 5 min and can be used for real-time detection of β-gal; the ratio gained an ultimate enhancement of about 260-fold. The ratio (F550/F450) displayed brilliant β-gal-dependent performance and responded linearly with β-gal activity. The probe showed wonderful biocompatibility and was successfully used for the bioimaging of endogenous β-gal in the human ovarian cancer cell line OVCAR-3.
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