Effects of biotic and abiotic factors on biofilm growth dynamics and their heterogeneous response to antibiotic challenge

Over the last couple of decades, with the crisis of new antimicrobial arsenal, multidrug-resistant clinical pathogens have been observed extensively. In clinical and medical settings, these persistent pathogens predominantly grow as complex heterogeneous structures enmeshed in a self-produced exopolysaccharide matrix, termed as biofilms. Since biofilms can rapidly form by adapting new environmental surroundings and have potential effect on human health, it is critical to study them promptly and consistently. Biofilm infections are challenging in the contamination of medical devices and implantations, food processing and pharmaceutical industrial settings, and in dental area caries, periodontitis and so on. The persistence of infections associated with biofilms has been mainly attributed to the increased antibiotic resistance offered by the cells growing in biofilms. In fact, it is well known that this recalcitrance of bacterial biofilms is multifactorial, and there are several resistance mechanisms that may act in parallel in order to provide an enhanced level of resistance to the biofilm. In combination, distinct resistance mechanisms significantly decrease our ability to control and eradicate biofilm-associated infections with current antimicrobial arsenal. In addition, various factors are known to influence the process of biofilm formation, growth dynamics, and their heterogeneous response towards antibiotic therapy. The current review discusses the contribution of cellular and physiochemical factors on the growth dynamics of biofilm, especially their role in antibiotic resistance mechanisms of bacterial population living in surface attached growth mode. A systematic investigation on the effects and treatment of biofilms may pave the way for novel therapeutic strategies to prevent and treat biofilms in healthcare and industrial settings.