Diallyl disulfide increases CDKN1A promoter-associated histone acetylation in human colon tumor cell lines
2006
Pecollo, Nathalie | Pagniez, Anthony | Thomas, Muriel | Cherbuy, Claire | Duée, Pierre-Henri | Martel, Paule | Chaumontet, Catherine, C. | Laboratoire de nutrition et sécurité alimentaire ; Institut National de la Recherche Agronomique (INRA) | Unité de recherche Nutrition et Sécurité Alimentaire (LNSA) ; Institut National de la Recherche Agronomique (INRA)
International audience
Mostrar más [+] Menos [-]Inglés. Diallyl disulfide (DADS) is an organosulfur compound from garlic, which inhibits colon tumor cell proliferation. In a previous study, we have shown that in Caco-2 and HT-29 cells DADS (200 microM) increases global histone acetylation, CDKN1A mRNA, and p21(waf1) protein levels and induces G2/M cell cycle arrest. These results suggested that DADS could inhibit cell proliferation through at least in part a transcriptional activation of CDKN1A expression involving histone acetylation. In this study, using chromatin immunoprecipitation assays, we demonstrate that in Caco-2 and HT-29 cells histone H4 and/or H3 acetylation is increased within CDKN1A promoter after 3 and 6 h treatments with DADS. These results strongly suggest that histone acetylation, a molecular mechanism implicated in the regulation of gene expression, could account for the induction of CDKN1A expression and the antiproliferating effects of DADS in colon tumor cells.
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