PCR: a sensitive diagnostic tool for Trypanosoma evansi in camels in Egypt
2024
Safeya Henidy | Somaia Abouakkada | Mohamed Bessat | Mohamed Elsokkary | Nadia Laban | Price Price | Adel El-Kattan | Ahmed L. Elnaggar | Mona Mahmoud | Amira Dewair
Camel Trypanosomiasis, or Surra, or El Debab as better known, caused by Trypanosoma evansi constitutes an economically important disease that affects the health and production of camels. Two-hundred and ninety-five samples from camels of different ages and sexes were collected from five geographic locations in Egypt (Behera, Cairo, South Sinai, Matrouh, Halayeb and Shalateen). Giemsa-stained smears that were prepared from blood samples were examined microscopically, while PCR coupled with DNA sequencing was applied for molecular detection and phylogenetic analysis. Microscopic and molecular findings revealed a prevalence of 0.34% and 50.51% in the examined camels through stained blood smears and PCR techniques, respectively. T. evansi is enzootic in Egypt, and the PCR technique could preferably be applied in surveillance studies as a more sensitive detection method.
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