The study of large scale Phalaenopsis amabilis cv. Detroit plantlets production through in vitro culture of the vegetative organs
2021
Balilashaki, Khosro | Zakizadeh, Hedayat | Olfati, Jamal-Ali
Orchids are one of the most popular plants in the world. Because of its hard propagation as most important problem, micropropagation technique has been employed recently. In this study, commercial micropropagation of orchid Phalaenopsis cut flower “Detroit” was done by vegetative tissue. First, nodal explants of Phalaenopsis amabilis cv. Detroit flower stalks cultured on Murashige and Skoog (MS) medium supplemented with different concentration of NAA and BAP for direct regeneration and the obtained planlets were cultured on ½ MS medium supplemented with different combination of NAA, IAA and activated charcoal for rooting. The sterile leaves of plantlets were cultured on ½ MS medium containing NAA, BAP and TDZ for evaluation of regeneration and direct production of somatic embryos. Results showed that effective concentration for plantlet regeneration obtained in MS medium containing 1 mg/l NAA and 4 mg/l BAP. The highest number of root produced in 2 mg/l NAA. The highest number of protocorms obtained at 3 mg/l TDZ. The plants survived rates from nodal flower stalk explants was 84.93%. At the end, the highest plantlets acclimatization (90.20 %) was in medium containing cocopeat and activated charcoal (1: 1).
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