Microbial community structure and activity in trace element-contaminated soils phytomanaged by Gentle Remediation Options (GRO)
2017
Touceda-González, M. | Renella, G. | Giagnoni, L. | Sessitsch, A. | Brader, G. | Kumpiene, J. | Dimitriou, I. | Eriksson, J. | Friesl-Hanl, W. | Galazka, R. | Janssen, J. | Mench, Michel | Muller, I. | Neu, S. | Puschenreiter, M. | Siebielec, G. | Vangronsveld, J. | Kidd, P.S. | Instituto de Investigaciones Agrobiológicas de Galicia (IIAG) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | Department of Agri-Food Production and Environmental Sciences ; Università degli Studi di Firenze = University of Florence = Université de Florence (UniFI) | Center for Health & Bioresources ; Austrian Institute of Technology (AIT) | Waste Science & Technology ; Luleå University of Technology = Luleå Tekniska Universitet (LUT) | Department of Crop Production Ecology ; Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Departement of Soil and Environment ; Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Austrian Institute of Technology (AIT) | Institute of Soil Science and Plant Cultivation (IUNG) | Centre for Environmental Sciences ; Hasselt University (UHasselt) | Biodiversité, Gènes & Communautés (BioGeCo) ; Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB) | Saxon State Office for Environment, Agriculture and Geology | Department of Forest and Soil Sciences ; Universität für Bodenkultur Wien = University of Natural Resources and Life Sciences [Vienne, Autriche] (BOKU)-Institute of Silviculture
International audience
Mostrar más [+] Menos [-]Inglés. Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe. Community structure was studied using denaturing gradient gel electrophoresis (DGGE) fingerprinting of Bacteria, α- and β-Proteobacteria, Actinobacteria and Streptomycetaceae, and sequencing of DGGE bands characteristic of specific treatments. The number of copies of genes involved in ammonia oxidation and denitrification were determined by qPCR. Phytomanagement increased soil microbial biomass at three sites and respiration at the Biogeco site (France). Enzyme activities were consistently higher in treated soils compared to untreated soils at the Biogeco site. At this site, microbial biomass increased from 696 to 2352 mg ATP kg-1 soil, respiration increased from 7.4 to 40.1 mg C-CO2 kg-1 soil d-1, and enzyme activities were 2-11-fold higher in treated soils compared to untreated soil. Phytomanagement induced shifts in the bacterial community structure at both, the total community and functional group levels, and generally increased the number of copies of genes involved in the N cycle (nirK, nirS, nosZ, and amoA). The influence of the main soil physico-chemical properties and trace element availability were assessed and eventual site-specific effects elucidated. Overall, our results demonstrate that phytomanagement of TECS influences soil biological activity in the long term.
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