23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores
2002
Delort, A.M. | Gaudet, Gérard | Forano, Evelyne | Université Blaise Pascal - Clermont-Ferrand 2 (UBP) | Unité de Microbiologie (MIC) ; Institut National de la Recherche Agronomique (INRA)
International audience
Mostrar más [+] Menos [-]Inglés. In order to measure intracellular sodium concentrations in resting cells of Fibrobacter succinogenes S85 by 23Na NMR spectrometry, two methodological aspects were studied. First, three different shift reagents (Dy(PPPi)7−2, Tm(DOTP)5−, and Dy(TTHA)3−) were tested for their ability to separate internal and external 23Na NMR resonances. Their toxicity toward F. succinogenes cells was evaluated by in vivo13C NMR experiments. Tm(DOTP)5− was found to be the most efficient shift reagent while being nontoxic. Second, a new methodology was developed to calculate intracellular sodium concentration in F. succinogenes by using ionophores. This approach avoided the problem of intracellular volume measurement and that of sodium visibility determination.
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