<i>Limosilactobacillus reuteri</i> and Its Probiotic Potential against Cariogenic Bacteria
2024
Brianda Karina Félix-Sicairos | Rita Elizabeth Martinez-Martinez | Saray Aranda-Romo | Roberto Gonzalez-Amaro | Mariana Salgado-Bustamante | Norma Verónica Zavala-Alonso | Ángel Gabriel Alpuche-Solís
Bacteriotherapy is a promising option in addressing dental caries, a persistent global public health challenge with multifactorial origin, including dysbiosis. Despite the exploration of various probiotics, outcomes remain inconclusive. Objective: This study aimed to assess the inhibitory potential of <i>L. reuteri</i> and other potential probiotics like <i>S. salivarius</i> and <i>S. oralis</i> on the growth, adhesion, colonization, and viability of major cariogenic pathogens, comparing their probiotic efficacy. Methods: An in vitro experimental study was conducted, encompassing direct competition assays in solid and liquid co-culture tests and the characterization of adhesion to dental enamel and cell viability by life or death assay. Results: <i>L. reuteri</i> exhibited the significant inhibition of <i>S. sobrinus</i> and <i>S. mutans</i> growth in both solid and liquid cultures, with statistically notable differences. Scanning electron microscopy and confocal microscopy demonstrated reduced cariogenic biofilm formation when combined with <i>L. reuteri</i>, corroborated by diminished bacterial viability and decreased dental enamel coverage. These findings underscore <i>L. reuteri’s</i> potential as an effective agent in caries prevention. Conclusion: The study suggests <i>L. reuteri</i> could serve as an effective probiotic in bacteriotherapy against dental caries. It displayed substantial inhibitory activity in vitro against cariogenic bacteria, impeding biofilm formation and adhesion, thereby impacting cell viability.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Directory of Open Access Journals