Identification of an immunogenic protein of Giardia lamblia using monoclonal antibodies generated from infected mice

Jael Quintero; Diana Carolina Figueroa; Rafael Barcelo; Linda Breci; Humberto Astiazaran-Garcia; Lucila Rascon; Ramon Robles-Zepeda; Adriana Garibay-Escobar; Enrique Velazquez-Contreras; Gloria Leon Avila; Jose Manuel Hernandez-Hernandez; Carlos Velazquez

Identification of an immunogenic protein of Giardia lamblia using monoclonal antibodies generated from infected mice

2013

The humoral immune response plays an important role in the clearance of Giardia lamblia. However, our knowledge about the specific antigens of G. lamblia that induce a protective immune response is limited. The purpose of this study was to identify and characterise the immunogenic proteins of G. lamblia in a mouse model. We generated monoclonal antibodies (moAbs) specific to G. lamblia (1B10, 2C9.D11, 3C10.E5, 3D10, 5G8.B5, 5F4, 4C7, 3C5 and 3C6) by fusing splenocytes derived from infected mice. Most of these moAbs recognised a band of ± 71 kDa (5G8 protein) and this protein was also recognised by serum from the infected mice. We found that the moAbs recognised conformational epitopes of the 5G8 protein and that this antigen is expressed on the cell surface and inside trophozoites. Additionally, antibodies specific to the 5G8 protein induced strong agglutination (> 70-90%) of trophozoites. We have thus identified a highly immunogenic antigen of G. lamblia that is recognised by the immune system of infected mice. In summary, this study describes the identification and partial characterisation of an immunogenic protein of G. lamblia. Additionally, we generated a panel of moAbs specific for this protein that will be useful for the biochemical and immunological characterisation of this immunologically interesting Giardia molecule.

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