Comparative Transcriptomics Reveal the Mechanisms Underlying the Glucosinolate Metabolic Response in Leaf <i>Brassica juncea</i> L. under Cold Stress
2023
Bing Tang | Bao-Hui Zhang | Chuan-Yuan Mo | Wen-Yuan Fu | Wei Yang | Qing-Qing Wang | Ning Ao | Fei Qu | Guo-Fei Tan | Lian Tao | Ying Deng
Glucosinolates (GSLs) are not only a unique flavor substance from leaf <i>B. juncea</i> but also a major secondary metabolite produced in response to abiotic stresses. Cold stress is one of the most common abiotic stresses in leaf <i>B. juncea</i>; however, the metabolic response pattern of GSLs in leaf <i>B. juncea</i> under cold stress has not yet been reported. In the present study, we analyzed the GSLs content of leaf <i>B. juncea</i> under cold stress and found that it increased and subsequently decreased. According to RNA-seq data, genes related to the synthesis of aliphatic GSLs were significantly upregulated following 24 h of cold stress; genes related to the synthesis of indole GSLs were significantly upregulated following 48 h of cold stress; and <i>BjBGLU25</i> and <i>BjBGLU27</i> were significantly upregulated. Further analysis of the correlation between transcription factors and GSLs content revealed that MYB, ERF, IQD, and bHLH may be involved in regulating the GSLs response pattern in leaf <i>B. juncea</i> under cold stress. In particular, an unreported transcription factor, BjMYBS3 (BjuVA05G33250), was found to play a possible role in the synthesis of aliphatic GSLs. And the external application of GSLs increased the ability of leaf <i>B. juncea</i> to cope with cold stress.
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