Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
2022
Tokuko Haraguchi | Takako Koujin | Tomoko Shindo | Şükriye Bilir | Hiroko Osakada | Kohei Nishimura | Yasuhiro Hirano | Haruhiko Asakawa | Chie Mori | Shouhei Kobayashi | Yasushi Okada | Yuji Chikashige | Tatsuo Fukagawa | Shinsuke Shibata | Yasushi Hiraoka
Haraguchi et al. investigate how transfected DNA is incorporated into the nucleus using light and electron microscopy, and the LacI/LacO system. The authors report that cytoplasmic localised plasmid DNA is incorporated into the nucleus as it reforms during M-exit and transgene expression was achieved only after nuclear reformation in the next G1.
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