Effect of genotypic background on haploid production through embryo rescue in wheat × maize crosses - Short Communication
2005
N. Singh | R.K. Behl | M.S. Punia
Two high yielding wheat (Triticum aestivum L.) varieties were pollinated with seven agrarian superior varieties. F1's thus obtained were pollinated with diverse winter maize lines/composites and treated with a solution containing 2,4-D and GA3. The haploid embryos were rescued 13 to 16 days post pollination on MS1 and MS4 media. Out of 15, only two crosses viz., (UP 2338 × HD 2285) F1 × maize and (WH 533 × PBW 343) F1 × maize resulted in callus development and direct germination via embryo rescue.
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