A Highly Sensitive Method for the Detection of Hydrolyzed Gluten in Beer Samples Using LFIA
2023
Segura Montero, Verónica | Siglez, Miguel Ángel | Ruiz Carnicer, Ángela | Martín Cabrejas, Izaskun | van der Hofstadt, María | Mellado Durán, María Encarnación | Comino Montilla, Isabel María | Sousa Martín, Carolina | Universidad de Sevilla. Departamento de Microbiología y Parasitología | Corporación Tecnológica de Andalucía (CTA)
Most gluten analysis methods have been developed to detect intact gluten, but they have shown limitations in certain foods and beverages in which gluten proteins are hydrolyzed. Methods based on G12/A1 moAbs detect the sequences of gluten immunogenic peptides (GIP), which are the main contributors to the immune response of celiac disease (CD). Immunogenic sequences with tandem epitopes for G12/A1 have been found in beers with <20 mg/kg gluten, which could be consumed by CD patients according to the Codex Alimentarius. Therefore, an accurate method for the estimation of the immunogenicity of a beer is to use two moAbs that can recognize celiac T cell epitopes comprising most of the immunogenic response. Here, a specific and sensitive method based on G12/A1 LFIA was developed to detect GIP in beers labeled gluten-free or with low gluten content, with an LOD of 0.5 mg/kg. A total of 107 beers were analyzed, of those 6.5% showed levels higher than 20 mg/kg gluten and 29% showed levels above the LOD. In addition, G12/A1 LFIA detected gluten in 15 more beer samples than competitive ELISA with another antibody. Despite their labeling, these beers contained GIP which may cause symptoms and/or intestinal damage in CD patients.
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