Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model

Castilla, Joaquín; Brun Torres, Alejandro; Díaz-San Segundo, Fayna; Salguero, F. J.; Gutiérrez-Adán, Alfonso; Pintado, Belén; Ramírez, Miguel Ángel; Del Riego, L.; Torres, Juan María; Gutiérrez-Adán, Alfonso; Díaz-San Segundo, Fayna [0000-0003-4439-3144]; Castilla, Joaquín [0000-0002-2216-1361]; Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]

Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model

2005

In this work we show evidence of mother-to-ofispring transmission in a transgenic mouse line expressing bovine PrP (boTg) experimentally infected by intracerebral administration of bovine spongiform encephalopathy (BSE) prions. PrPres was detected in brains of newborns from infected mothers only when mating was allowed near to the clinical stage of disease, when brain PrPres deposition could be detected by Western blot analysis. Attempts to detect infectivity in milk after intracerebral inoculation in boTg mice were unsuccessful, suggesting the involvement of other tissues as carriers of prion dissemination. The results shown here prove the ability of BSE prions to spread centrifugally from the central nervous system to peripheral tissues and to offspring in a mouse model. Also, these results may complement previous epidemiological data supporting the occurrence of vertical BSE transmission in cattle. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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