The first genetic linkage map of sea buckthorn (Hippophae rhamnoides) and QTL mapping of growth-related traits

Geng, R.M.; Han, Y.Z.; Liu, Z.H.; Xie, Q.

The first genetic linkage map of sea buckthorn (Hippophae rhamnoides) and QTL mapping of growth-related traits

2024

Geng, R.M. | Han, Y.Z. | Liu, Z.H. | Xie, Q.

Sea buckthorn (Hippophae rhamnoides), a hardy deciduous shrub of the family Elaeagnaceae, is known for its economic and ecological importance due to its wide use in medicine, food, windbreaks, and sand fixation. In this study, the first genetic linkage map of H. rhamnoides was constructed based on an F1 population (H. rhamnoides subsp. mongolica ‘wulangemu’ × H. rhamnoides subsp. sinensis ‘wucixiong’) by using start codon targeted (SCoT), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) markers. The linkage map contained 1143 markers with a total length of 1889.46 centimorgans (cM), including 12 linkage groups (LG) and covering 97.17% of the estimated genome, with an average marker density of 1.67 cM. According to the phenotypic variation analysis, 61 QTLs were identified. Among these QTLs, there were 18 QTLs for seedling height, 27 QTLs for ground diameter, two QTLs for primary branching angle, 11 QTLs for leaf width, and 3 QTLs for leaf shape index. The range of phenotypic variation explained by these QTLs was 10.7 ~ 21.9%. The QTLs identified by high-density linkage mapping reported in this study will be useful for the molecular breeding of sea buckthorn.

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