Molecular detection of Methicillin-Resistant Staphylococcus aureus isolated from Subclinical Mastitis Cows in Basrah Province.
2025
Zainab Abdulameer Farhan, Nawres Norri Jaber, Rana Adnan Fayez.
Out of 200 milk samples collected from apparently normal cows in Basrah province from 21 November 2023 to 6 February 2024, 95 (48)% were positive for the CMT test. This study used various techniques to detect the presence of staphylococcus aureus, including conventional microbiological assays (mannitol salts agar and CHROMagar™ Staph aureus) and molecular methods (amplifying the nuc gene using polymerase chain reaction ). The percentage of S. aureus isolates was 38 (19 % ).From 38 ( 19 %) S. aureus isolates, 27 (71%) carried the mecA gene, 6 (16%) carried pvl, while none of the isolates carried the mecC gene.
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