Evaluation of different staining techniques for detection of Cryptosporidium oocysts in backyard chickens ((Gallus gallus domesticus))
2025
Nadia Mohammed | Abdullah Abdullah
In this study, the parasite Cryptosporidium spp. was investigated in backyard chicken and the effect of age and sex on the infection rate was studied. All samples taken from feces and intestinal contents were examined microscopically by staining methods including modified acid-fast stain, Malachite Green Stain at a concentration of 5%, 1%, Nigrosin Stain (1%), and Safranin methylene blue staining. The overall infection rate was 48.7%. The Oocyst appeared in an oval to spherical shape with a bright red color in modified acid-fast stain and without stain with a green background in malachite green stain 5% and light malachite green 1%. The oocyst appeared brighter, while in an orange color when using safranin methylene blue stain. The highest infection rate was found in the modified acid-fast stain (48.7%), Its efficiency reached 100%, Nigrosin stain (47%), malachite green stain (45.2%), and safranin blue stain (43.5%), while the lowest infection rate in the light malachite green stain (30.4%). The oocysts were divided into two types of cysts depending on the size of the cysts (large cysts and small cysts) and on the wall thickness (thick-walled cysts and thin-walled cysts). the highest infection rate was in young ages less than three months (60.8%), while in adults, the infection rate was 29.2%, no significant difference was recorded between the infection rate in females and males,
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