MOLECULAR IDENTIFICATION OF (EfaA) IN ENTEROCOCCUS FECALIS AND ENTEROCOCCUS FACIUM AND THEIR ROLE IN BIOFILM FORMATION
2016
Ashwak B.J. Al-Hashimy | Aya H. Alhalaby
Total of (104) urine samples were collected from patients suffering from urinarytract infection with different age groups from five hospitals in Baghdad (Ibn-Albalady, Al Yarmouk, Medical city, Baghdad hospital and Al-Kandy) from theperiod of the beginning of September 2015 to the end of December 2015.All sampleswere examined by traditional methods based on cultural characteristics, biochemicaltest and API 20 strep. The results revealed 50 isolates to Enterococcus and this wasconfirmed by polymerase chain reaction technique (PCR) based on amplification ofspecies specific genes. PCR were performed for E.faecalis and E.faecium in order toconfirm the presence of EfaA genes which code for Enterococcus faecalisendocarditis antigen using specific primer for gene.The results showed thatEnterococcus contain a proportion of 100% of EfaA.Biofilm production was detectedin E.faecalis and E.faecium by using two methods: Congo red agar method andmicrotiter plate method.Our results show that22(44%) of Enterococcus isolates werestrong biofilm production,25(50%)as moderate and 3(6%) as week biofilm productionby use Congo red method.In microtiter plate method, our results show that 20(40%) ofbacterial isolates were detected as strong, 26(52%) as moderate and 4(8%) as weekbiofilm production. This study aims todiagnosis of E.faecalis and E.faecium fromurinary tract infection of patients by traditional and molecular methods, detection ofEfaA gene and its role in biofilm production.
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