DETECTION OF bla-VIM1GENE IN CARBAPENEMRESISTANT Pseudomonas aeruginosa ISOLATED FROM CLINICAL SAMPLES IN WASIT PROVINCE HOSPITALS
2018
Zeyad Khalaf Hussein | Israa Jabbar Shamkhi
The present study investigated the presence of bla-VIM1in clinical isolates ofPseudomonas aeruginosa. During the period from November 2017 to February 2018,a total of two hundred patients admitted to (Al-Zahraa Teaching Hospital, Al-KaramaTeaching Hospital and Al-Kut Hospital for Gynecology, Obstetrics and Pediatrics) inWasit province. One hundred and three of isolates were diagnosed as P. aeruginosa.High prevalence of P. aeruginosa isolates were detected in burn swab samples 70(35%) followed by sputum 12 (6%) and ear 11 (5.5%). All 103 P. aeruginosa isolateswere primarily screened for carbapenems - resistance, 36 (34.95%) were resistant tocarbapenems. Carbapenems resistant isolates were underwent antimicrobialsusceptibility to 14 antibiotics using Kirby-Bauer disk diffusion method. Thephenotypic and molecular methods of carbapenem resistance were investigated.Carbapenems resistant isolates were identified by the Double disc synergy test, whichdetects the probability of isolates able to produce Metallo-beta- lactamases (MBL).Out of the isolates 32 (88.89%)were positive. Imipenem-EDTA combined diskmethod showed 30 (83.33%) of isolates possessed ability to produce the Metallobeta-lactamases. In addition , the Modified Hodge test (MHT) showed the ability ofisolates to produce Carbapenemases enzyme. 16 (44.44%) of the isolates werepositive to Modified Hodge test. Carbapenemase gene were detected by PCRtechnique. The results demonstrated that out of 36 carbapenems resistantPseudomonas aeruginosa (CRPA) isolates 34 (94.44%) were positive to bla-VIM1gene.
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