MOLECULAR DETECTION OF TETRACYCLINE RESISTANCE GENES IN Bacillus cereus ISOLATED FROM FOOD SOURCES
2018
Ban M.S. Saeed | Basil A. Abbas | Shaker A.N. Al-jadaan
Two hundreds of different food samples i.e. cream, beef meat, frozen beef meat, burger,cooked rice and minced rice were collected from local markets in Basrah city. The testedsamples of each food type were found to be contaminated with Bacillus cereus (B. cereus) in aratio of 36.36 % for cream, 26.47 % for beef meat, 50 % for frozen beef meat, 36.36 % forburger, 18.18 % for cooked rice and 51.51 % for minced rice. Mannitol egg-yolk agar (MYP)supplemented with polymyxin B sulfate is a selective media used for isolation. Identification ofthe isolate was done by detection of 16SrDNA and conformed by sequencing. Antimicrobialsusceptibility test was used for screening the isolates, which were resistance to tetracycline.Bacillus cereus isolates were resistance to tetracycline TE 30 mg/disc (100 %) in cream and (0%) in cooked rice. Polymerase chain reaction was used for detection of the presence oftetracycline resistance gene in the percentage 32 % and 44% for tet (K) and tet (L), respectively.
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